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Ultimately, GLP-1 was identified as a potential incretin hormone (89, 91)
Semaglutide slows down bowel activity, implying that food stays longer in the stomach, thus enhancing glycemic control as well as appetite and craving
The bound Fab was eluted with 25 mM sodium citrate, pH 3.2 and pooled for further purification by size-exclusion chromatography on a Superdex75 column (GE Healthcare) in phosphate buffered saline (PBS)
Taking one 30 minutes before a meal can prevent reflux in predictable trigger situations
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