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Sections were permeabilized with 0.3% Triton X-100 for 3 h, blocked with 5% FBS in PBS containing 0.1% Tween 20 for 1 h, and incubated overnight at 4 C with primary antibodies (anti-glucagon, Abcam ab92517
In April 2024, a year after starting my GLP-1 journey, I was down 133 pounds
Additional discussions, including comparisons with FDA drug labels and major clinical guidelines, are presented in Supplementary Materials 4
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