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By identifying the concentration and accurately performing calculations, you can avoid potential pitfalls associated with incorrect dosing

Participants had existing cardiovascular disease and a BMI of 27 or higher but did not have diabetes

Initially (untreated cells for initial time point) or after 4 days (with varying treatment conditions), 40 L of Cell Titer Glo reagent (Promega) was added to each well, mixed briefly, and the luminescence read on a luminometer (Molecular Devices)

Iron status: Consider screening for iron deficiency with a complete iron panel (ferritin, serum iron, TIBC, transferrin saturation), particularly in menstruating women