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Epitalon is particularly useful in models exploring cellular lifespan dynamics, signaling efficiency, and peptide-driven regulatory balance, especially in environments designed to evaluate long-term cellular behavior and adaptation

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The second position of its N-terminus is glycine (alanine in GLP-1), which is not easily degraded by the DPP-4 enzyme

The supernatant was then removed, replaced with 0.5 mL of 4% paraformaldehyde solution, and incubated at room temperature for 15 min