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The theory holds that high lactic acid buildup makes IGF-1 receptors more receptive, so injecting into muscles about to be trained maximizes local effects

taken orally as a mixed powder, capsule, or ready-to-drink dose, generally used continuously or in 8-12 week cycles to assess effect Pros: Widely available, inexpensive, and legal over-the-counter with no prescription needed Strong safety track record from decades of dietary use Supported by multiple randomized controlled trials for skin hydration/elasticity and joint comfort Cons: Effect sizes are often modest and some trials show outcomes close to placebo Requires weeks to months of consistent daily use before noticeable benefit Not a substitute for injury-specific treatment in acute or severe injuries Side Effects: Generally well tolerated

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Cell viability assay The viability of cells was determined by using crystal violet staining solution