Figure 5 In order to determine if the phosphorylation of Ser42, Ser184, and Tyr198 was involved in the effect of GSTP on HMGB1, six GSTP mutants were constructed, including GSTP-S42A (a Ser42 non-phosphorylatable mutant), GSTP-S42D (a Ser42 constant-phosphomimetic mutant), GSTP-S184A (a Ser184 non-phosphorylatable mutant), GSTP-S184D (a Ser184 constant-phosphomimetic mutant), GSTP-Y198F (a Tyr198 non-phosphorylatable mutant), as well as GSTP-Y198D (a Tyr198 constant-phosphomimetic mutant) (Figure S4B in Supplementary Material)
19 Early studies: There were studies in the 1950s and the1960s that showed that oral vitamin B 12 could be absorbed by patients with pernicious anemia and could lead to resolution of the anemia
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