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three random samples for each experimental condition were used for microarray analysis and all the four samples for quantitative polymerase chain reaction (qPCR) validation
Quick Answer To reconstitute a lyophilized peptide, disinfect both vial stoppers with alcohol, slowly draw the desired volume of bacteriostatic water into a sterile syringe, inject the water gently down the inside wall of the peptide vial (avoiding direct impact on the powder whenever possible), allow the powder to dissolve naturally, gently swirl if needed, inspect the solution for clarity, and refrigerate according to recommended laboratory storage conditions
Heymsfield SB, Greenberg AS, Fujioka K, et al
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