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Briefly, the construct was produced by stable expression and secretion from CHO-K1 cells and then purified by a sequential combination of 9E10 antibody-affinity chromatography and size-exclusion chromatography as previously described 35 , with the final sample being prepared at a concentration of 0.2 mg mL 1 in a 24.8 mM Tris-HCl (pH 8.0), 137 mM NaCl, 2.7 mM KCl plus 0.02% NaN 3 (TBSA)

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Koi sits in the upstream research-supply category instead, dispatching a lyophilized analytical-grade peptide with COA documentation for bench-side in vitro work, with no patient-directed preparation involved

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