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The participants were placed in different conditions to promote a regular sleep schedule, sleep deprivation in the dark, sleep deprivation with light exposure at night, and sleep deprivation with filtered light exposure at night

Unlike standard IGF1, the LR3 version has two key modifications that enhance its potency and duration: The Long designation refers to an additional 13 amino acids, increasing its molecular stability The Arg3 substitution reduces its binding to IGF-binding proteins (IGFBPs), extending half-life from minutes to ~2030 hours These changes allow IGF1 LR3 to remain active in the bloodstream much longer than endogenous IGF1, resulting in greater anabolic signaling and sustained muscle growth potential

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