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Under oxidizing conditions, the demand for glutathione increases and GCL is activated
Due to sperms abundance of membrane polyunsaturated fatty acids (PUFAs) and their own innate capacity to generate reactive oxygen species (ROS) (via abnormally shaped sperm morphology), human sperm are highly susceptible to oxidative stress (inflammation)
cGAMP analysis via ELISA B16-GM cells (5 10 5 ) were resuspended in 250 l RIPA lysis buffer (Pierce catalogue number 89901) supplemented with cOmplete Protease Inhibitor Cocktail (Sigma catalogue number 11697498001) and lysed with a 28 gauge needle
Nature Reviews Immunology, 21 (2), 7382
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