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Samples were then analyzed by gas chromatography-mass spectrometry (GC-MS) with electron capture negative ionization
It is a natural occurring enzyme in every cell throughout the body that has a pivotal function in energy metabolism (storing and transfer)
The media was then replaced with glucose-free DMEM supplemented with 2% dialyzed FBS and 25 mM [U- 13 C]-glucose (Cambridge Isotope Laboratories, CLM-1396, 99% atom 13 C) for either 30 min or 2 h, or [U- 13 C]-palmitate (Cambridge Isotope Laboratories, CLM-409, 99% atom 13 C) for 24 h
Third-Party Tested: Independent HPLC and mass spectrometry verification not self-reported purity claims
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