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Transporter localization was disrupted in patient DhLOs, as indicated by immunostaining (Fig
The rapid withdrawal of corticosteroid therapy can induce to a secondary renal failure
Cell lysis and protein extraction For protein extraction, cells were disrupted with a cell scraper in a lysis buffer consisting of 50 mM HEPES-Na, pH 7.2, 0.5 M NaCl, 0.5% (v/v) NP-40, 0.05% LDS and 1Pierce EDTA-free protease inhibitor cocktail (Thermo, Australia) in Milli-Q H 2 O and then vortexed
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