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Different reconstitution volumes produce different concentrations from the same vial: 50mg vial + 2ml water = 25mg/ml (very concentrated) 50mg vial + 2.5ml water = 20mg/ml (concentrated) 50mg vial + 5ml water = 10mg/ml (standard) 50mg vial + 10ml water = 5mg/ml (dilute) Higher concentrations require smaller injection volumes but may exceed solubility limits for some peptides

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At the second step, a second H 2 O 2 molecule acts, as a reducing agent, on Compound I, producing one molecule of water, one of O 2 and the enzyme in the resting state [45, 50, 52]