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The three libraries were generated using error-prone PCR as described above, using the following conditions to produce varying levels of mutagenesis: Library 1: 2 M 8-oxo-dGTP, 0.5 M dPTP, 20 PCR cycles Library 2: 2 M 8-oxo-dGTP, 1 M dPTP, 20 PCR cycles Library 3: 2 M 8-oxo-dGTP, 2 M dPTP, 20 PCR cycles The library sizes (approximated by number of transformants as described above) were 6 10 7 for each library
Considering the promising results of the SURPASS and SURMOUNT trials, what additional factors or challenges must researchers address before adopting tirzepatide for conditions beyond Type 2 diabetes, such as non-alcoholic fatty liver disease (NAFLD)
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Am J Biomed Sci Res
