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After completing a 14-day cycle, a break of one to three weeks is recommended before any repeat application [20]

For the fed-batch culture in the 5-l bioreactor, medium containing 15 g/L Na 2 HPO 4 12H 2 O, 3 g/L KH 2 PO 4 , 0.5 g/L NaCl, 3 g/L NH 4 Cl, 0.2 g/L MgSO 4 7H 2 O, 0.011 g/L CaCl 2 , 0.5 mL/L vitamin B1 (1 % w/v), 0.5 mL/L of a stock solution of trace elements, and 4 g/L glucose was used

Blankenberg S, Rupprecht HJ, Poirier O, Bickel C, Smieja M, Hafner G et al

Subsequently, an immobilon western chemiluminescent HRP substrate (Merck Millipore, Darmstadt, Germany) was used for detection in accordance with the manufacturers instructions