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At the end of all incubations, the hECs were washed three times with ice-cold PBS containing 0.1% BSA, once with acid stripping buffer (glycine 50 mM, NaCl 100 mM, pH 3) to remove the non-internalized, but cell surface-attached nanocarriers, and finally with PBS
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light intensity 75 mol m 2 s 1 ), were floated on liquid MS media supplemented with 100 M MTX for 24 h prior to harvesting
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