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Therefore, affinity-purified rEE2 eluates and rMCP insoluble fractions were further subjected to size exclusion chromatography (SEC) purification: For rEE2, suitable eluates were further purified using Superdex 200 increase 10/300 or 16/600 columns (Cytiva, Little Chalfont, UK) on a GE AKTA Explorer system (GE Healthcare)

doi:10.1016/j.clinbiochem.2003.08.004

He also separated stress into two types such as acute (short-term) and chronic (long-term), depending on how long the stressful event lasted (131, 132)