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Losing more than roughly 1% of bodyweight per week past month one
Eventually, this results in chronically insufficient GSH levels to neutralize the ROS, leading to oxidative stress that damages lens proteins
Slides were mounted with Fluoromount-G (Southern Biotech, USA) and examined using a Leica TCS SP5 AOBS confocal laser scanning microscope (Leica Microsystems, Germany) equipped with HCX PL APO 63 oil (NA = 1.4) objectives
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