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Where necessary, membranes were stripped using Restore reagent (Thermofisher, UK) then reprobed for the housekeeping gene GAPDH (1:2000 Cat# MCA4740, Bio-Rad, UK) as a loading control
Investigated as a research pairing because the two peptides are studied at largely non-overlapping targets, allowing combined exploration of growth-factor and cytoskeletal pathways in the same model
MOTS-c optimizes mitochondrial function and metabolic efficiency through AMPK activation, improved insulin sensitivity, and support for mitochondrial biogenesis
Often referred to as the master antioxidant, glutathione is essential to protect all cells, tissues and organs form oxidative fallout and damage
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