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While their approach successfully employed LC-MS for metabolic profiling, it relied on in vivo 15 N uniform metabolic labeling to achieve accurate quantification
In brief, HUVECs were lysed in Co-IP buffer containing 20 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.5 mM EDTA, 0.5% NP-40, 0.5 mM PMSF, and 0.5% protease inhibitor cocktail on ice for 20 min
Activation of GLP-1 receptors in cortical arterioles enhances cerebral blood flow and improves perfusion, which may promote neuroprotection against ischemic stroke [44]
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