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This makes the math simple and reduces the chance of making a measurement error
It is synthesised in the cytosol by glutamate-cysteine ligase (EC 6.3.2.2) and glutathione synthetase, with the first, rate-limiting step feedback-inhibited by glutathione itself and intracellular concentrations typically 1 to 8 mM (Griffith, Free Radical Biology and Medicine, 1999)
A post-mortem study by Tang et al
Understanding how that diluent interacts with vial size, blend composition, and storage conditions matters more for research accuracy than any single fixed ratio and thats the framework this guide walks through
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