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Furthermore, when the ratios of NAD + to NADH in wild-type and mutant strains were compared we detected a significantly lower ratio of NAD + to NADH in the gdh3/gdh3 mutant than in the wild-type strain when incubated in proline medium, while a higher ratio of NAD + to NADH in the gdh3/gdh3 mutant than in the wild-type strain in MM medium (Supplementary Fig

The morphological characteristics include rupture of mitochondrial cristae, decrease or absence of mitochondria, degradation of the mitochondrial outer membrane, and mitochondrial shrinkage [53,54]

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Although some treatments can relieve symptoms in the short term, their overall effectiveness is still limited by a number of factors