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GPX4-IN-3 (26a) exhibits IC50 values of 0.78 M, 6.9 M, 0.15 M and 4.73 M in 4T1, MCF-7, HT1080 and HT1080 (with Fer-1) cells, respectively[1].GPX4-IN-3 (26a) exhibits outstanding GPX4 inhibitory activity with a percent inhibition up to 71.7% at 1.0 M compared to 45.9% of RSL-3[1].GPX4-IN-3 (26a) could significantly induce lipid peroxide (LPO) increase and effectively induce ferroptosis with satisfactory selectivity[1].GPX4-IN-3 (26a) is more likely to induce ferroptosis through the accumulation of intracellular peroxides via inhibiting GPX4 activity[1].GPX4-IN-3 (26a) significantly increased the level of ROS in 4T1 cells, which could also be reversed by Ferrostatin-1 (fer-1)[1]

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