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Reconstitution Process: Allow the peptide vial and bacteriostatic water to reach room temperature Using a sterile syringe, draw the desired volume of bacteriostatic water (typically 2.5 mL for a 5 mg vial) Inject the water slowly against the vial wall, allowing it to run down gently

Following lysis, the cell lysates were centrifuged at 12,000 g for 5 min at 4 C, and the supernatant was collected for further analysis

Dose, route, and cycle length change with the injury, the tissue, and what else you're taking