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In this case, a person may benefit from supplementing with the active form of folate
Single-cell transcriptomics of the mouse kidney reveals potential cellular targets of kidney disease
After 24 h, cells were washed twice with cold PBS and lysed in immunoprecipitation buffer (50 mM Tris-Cl, 150 mM NaCl, 5 mM EDTA, and 1% NP-40, pH 7.8) containing 1 protease inhibitor cocktail (Sigma) on ice for 1 h
the frontal lobe(Harada et al., 2011)
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