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In order to investigate whether elevated levels of reduced GSH were due to less redox active heme iron being liberated from impaired hemoglobin uptake and digestion in resistant parasites, 24 hpi trophozoite-stage parasites were treated for 3 h with 10 M of the cysteine protease inhibitor, E64d, to block hemoglobin digestion and decrease free heme levels
It addresses five hallmarks of aging at once: telomere maintenance, epigenetic regulation, antioxidant defense via Nrf2, immune recalibration, and circadian rhythm restoration
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