It exerts systemic effects, benefiting organs and tissues throughout the body
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Just a year-and-a-half later, though, Magness left, thoroughly disillusioned with a program that pushed into the gray area of medicating athletes to gain an advantage, and one he came to believe had crossed the line into outright doping
After incubation with the appropriate antibodies for 30 min, erythrocytes were lysed with lysis buffer (BD FACS Lysing Solution, BD Biosciences, San Jose, USA) for 10 min on a shaker, followed by centrifugation (250 x g, 5 min, 4C, Centrifuge 5427 R, Eppendorf SE, Hamburg, Germany) and washing with HBS