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Steady-state kinetic analysis showed that Arabidopsis GS uses a random Ter-reactant mechanism with binding of either ATP or -glutamylcysteine preferred first and addition of glycine last (Jez and Cahoon, 2004)
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Cells were lysed with 500 L of RIPA lysis buffer containing 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 25 mM Tris-HCl, supplemented with protease and phosphatase inhibitors at a 1:100 dilution
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