indeed, if some studies showed that the hormone is able to significantly downregulate autophagy in different disease models 63,64,65 , other reports failed to confirm those findings, providing evidence for an enhancement of the autophagic process upon melatonin treatment 66,67,68
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Surgically isolated tissues from four patients with ovarian cancer were minced, digested with 0.25% trypsin (Gibco, Gaithersburg, MD, USA), and centrifuged at 1500 r/min for 5 minutes