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Needle gauge matters too: a finer needle (commonly 29-31 gauge for insulin syringes) reduces the mechanical stress placed on the solution as it passes through the needle bore compared to a larger-bore needle, and produces a smaller, cleaner puncture through the vial's rubber stopper, reducing the risk of coring small rubber fragments breaking off into the solution

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A high HPLC purity value alone does not prove biological activity, sterile suitability, endotoxin status, clinical safety, or appropriateness for human or veterinary use

This skill takes good training to learn and plenty of hands-on practice to master, but it is one of many things taught to students in a medical assisting program like the one at UEI