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As an eighth example, lysine has a predominant positive net charge below pH~10.0, thus for cation exchange the pH elution gradient with a solvent comprising an organic modifier could begin below pH 10.0 to elute a target GLP-1 moiety acylated in the side chain of the lysine residue and end the gradient above pH 10.0 thereby subsequently removing an undesired non-acylated form

doi: 10.3389/fcvm.2023.1268814 145 EmaniSMPiekarskiBLHarrildDDel NidoPJMcCullyJD

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