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Description

Preparation of tung oil-conditioned medium Tung oil (Sigma, 440337)-conditioned medium was prepared by combining tung oil and RPMI-1640 complete medium in a 1:2 ratio and emulsifying the components by rotating for 45 min at room temperature

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1A), but with differences noted in EC 50 values for GEP44 (492.6 pM), GEP12 (17.3 nM) and Ex-4 (28.7 pM) at the GLP-1R (Fig

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