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With the GSH-ee experiments, mice were injected intraperitoneally with either vehicle (saline) or Glutathione ethyl ester (Cayman Chemical, 14953) for 4 days ((17-20) days post AAV-TBG-Cre treatment)
pSSBR was then treated with 10 units of Nb.BtsI (NEB) at 37 C for 4 h, generating a plasmid with single-strand breaks (nicks) on opposing strands, separated by a distance of 36 bp
Serious adverse events are rare but can happen
You can also find vitamin B12 in fortified cereals and nutritional yeast products
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