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The authors argue that their data indicate that isoprenaline acts on a pool of cAMP that directly acts on its target protein (hyperpolarization-activated cyclic nucleotide gated channels, HCN) in the rat sinus node to raise the beating rate (Merino et al

Bac water contains high-purity WFI water and 0.9% benzyl alcohol, commonly used in research environments

mRNA levels were determined by qPCR with SsoAdvanced Universal SYBR Green Supermix (Bio-Rad cat#1725270) using Rotor-Gene Q (Qiagen)