The sensor fluorescence response was measured over a time course and an oxygen ramping script mode together with the ACU was used to apply an oxygen gradient by flushing the measurement chamber with nitrogen gas (99.998 % v/v, Westfalen AG, Mnster, Germany)
302 Moreover, inulin, a polysaccharide dietary fiber, can enhance the effectiveness of anti-PD-1 therapy by increasing the abundance of beneficial commensal microbiota genera (e.g., Akkermansia , Lactobacillus and Roseburia ) and SCFAs, further increasing the number of stem-like T-cell factor-1 (Tcf1) + PD-1 + CD8 + T cells

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