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The cells were treated with 10 mM EtGSH in FluoroBrite DMEM for 34 h, 1 mM BSO in FluoroBrite DMEM for 34 h, 1 mM H 2 O 2 for 20 min in FluoroBrite or vehicle (FluoroBrite DMEM)

Washed beads were resuspended in 100 L of 50 mM HEPES buffer (pH 7.4) and sequentially reduced with TCEP (200 M) and re-alkylated either with IAM (400 M) Procedure A or with IAM-TPP (400 M) Procedure B for 30 min at RT in the dark

The mechanism is not well established it may relate to individual sensitivity, dehydration coinciding with supplement use, or other unrelated factors

Es especialmente beneficiosa para las personas mayores cuya capacidad de produccin de glutatin ha disminuido y ayuda a reactivar la accin mitocondrial, aumentando as su metabolismo