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The growth medium was removed after treatment, and the cells were cultured in 1x MTT (Keygen Biotech, catalog KGA311) in fresh growth medium for 4 h
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Mistake 5: reconstituting with the wrong water Bacteriostatic water contains 0.9% benzyl alcohol as a preservative, which allows the reconstituted solution to remain stable and sterile for multiple uses over days or weeks
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