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The cells were then collected and washed, followed by quantification of cellular levels of Cystine-FITC by flow cytometry analysis
The samples were transferred quantitatively onto spin columns (BioEcho) and washed as follows: 0.4% SDS in PBS (3 700 l), 6 M urea in ddH 2 O (2 700 l) and PBS (3 700 l) and transferred into new 1.5-ml LoBind tubes (Eppendorf) with 2 100 l X buffer (7 M urea, 2 M thiourea in 20 mM HEPES, pH of 7.5)
Step 3 Activate & Treat Apply the 1% AHK-Cu Treatment Spray directly to the scalp
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