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Protein and lipid pellets were discarded, while 10 L injections of supernatants were analyzed via a Vanquish UHPLC system (Thermo Fisher Scientific) coupled to a Q Exactive mass spectrometer (Thermo Fisher Scientific) utilizing an untargeted acquisition as previously reported 18

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Systemic GLP-1R agonist treatment reverses mouse glial and neurovascular cell transcriptomic aging signatures in a genome-wide manner

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instead, we roll it gently until the powder dissolves

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