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For fluorescence staining, we incubated samples for 2 h with Alexa-488 and Alexa-594 conjugated donkey antibodies (1:200) against mouse and rabbit immunoglobulins (Supplementary Figs 6 and 7 are representative images from two sets of immunolabelled cultures of NPCs and neurons)

The result of his insight would eventually reshape the treatment of metabolic disease worldwide, leading to a class of drugs that now includes Ozempic, Wegovy, Mounjaro, and Zepboundmedications that have been called the most important advance in metabolic health since insulin itself

Sample m/z range was set to 3004000 Da