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Though melasma is complex, there are well known common triggers: heat, stress, ultraviolet (UV) radiation exposure, and hormonal fluctuations (particularly estrogen)

Metformin also reduces NLRP3 inflammasome activation in macrophages by promoting autophagic clearance of damaged mitochondria (119, 120)

In two human cases of chronic elemental mercury exposure, use of unithiol 600 mg/day orally resulted in peak urine mercury excretion of 2000 g/L [Citation90], while quadrupling the dose resulted in peak urine mercury concentrations of only 1500 g/L [Citation57]

Cell cultures were conducted under sterile conditions using a laminar flow hood