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Expression and Purification of Glutathione S-Transferase (GST)-Tagged Recombinant C3a.1 The cDNA sequence encoding grass carp C3a.1 was amplified by PCR using the primer pair exC3a.1-F/exC3a.1-R listed in Supplementary Table 1

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Tanaka H, Takano K, Iijima H, Kubo H, Maruyama N, Hashimoto T, Arakawa K, Togo M, Inagaki N, Kaku K