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Extracts from Decalepis hamiltonii delayed early onset of PD symptoms, improved circadian rhythm and motor activity in PD model flies possessing A30P and A53T -synuclein mutation which are known to cause accelerated age dependent deterioration of circadian rhythm 29

Establish a systematic rotation pattern, such as: Week 1: Right abdomen Week 2: Left abdomen Week 3: Right thigh Week 4: Left thigh Week 5: Right upper arm Week 6: Left upper arm Week 7: Return to right abdomen This approach ensures each site receives adequate recovery time between injections

1a) [44, 47]

Histological, immunohistochemical, and fluorescence staining After euthanizing the animals, skin lesions without marginal tissue were removed and fixed in a paraformaldehyde fixative (Servicebio) for 12 h