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Primer sequences used for qPCR are listed below: Gsr Fwd: GTTTACCGCTCCACACATCCTG Rev: GCTGAAAGAAGCCATCACTGGTG Gclm Fwd: TCCTGCTGTGTGATGCCACCAG Rev: GCTTCCTGGAAACTTGCCTCAG Nqo1 Fwd: GCCGAACACAAGAAGCTGGAAG Rev: GCAAATCCTGCTACGAGCACT Srxn1 Fwd: TACCAATCGCCGTGCTCATCCG Rev: CCTTTGATCCAGAGGACGTCGA Hmox1 Fwd: CACTCTGGAGATGACACCTGAG Rev: GTGTTCCTCTGTCAGCATCACC Hprt Fwd: CAAGCTTGCTGGTGAAAAGGA Rev: TGAAGTACTCATTATAGTCAAGGGCATATC The volume of each reagent used in a 20 ul qPCR reaction is listed below: The setting for the cycling time for the qPCR reaction is listed below: Statistical analysis
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