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The elution gradient was optimized as follows: started from 10% B and increased to 70% B over 5 min

The cyclic structure and the D-residue introduce their own verification questions: mass spectrometry confirms composition but cannot distinguish a D-phenylalanine from an L, since the two are isobaric, and cyclization changes mass by only the loss of water relative to the linear precursor

Our previous research demonstrated that U0126 can inhibit the ERK pathway by reversing the induced levels of pERK1/2 in PFOS-treated RTCs 3

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