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Briefly, sodium bicarbonate, glucose, sodium pyruvate, and all 20 amino acids (except cystine and serine), biotin (B4639, Sigma), and vitamin B12 (V6629, Sigma) were added to the DMEM medium (#D9800-28, USBiological, Salem, MA) to match standard IMDM, supplement with 15% human AB serum and 15% dialyzed FBS (#SH300790340, Cytiva Hyclone), along with 10 ng/mL interleukin-3, 50 ng/mL stem cell factor and 2IU/mL erythropoietin
I find that its easier to hit my other goals if I eat more carbs and fiber and less fat (since it doesnt offer micronutrients with high calories)
If Calocurb is taken one hour before a meal, the natural response to eating a meal will follow, with an enhanced appetite suppression that is slightly prolonged but gradually tapers off
The staged approach may feel unfamiliar or inconvenient, but it's the difference between comfortable eating and spending your evening feeling miserable
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