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The left ICA was temporarily clamped and an incision made on the CCA to allow the insertion of a 60 suture with a silicone-coated tip (602223PK10RE, Doccol, USA)
Supplementation of this vital nutrient can be successful but its far from straightforward as glutathione is unstable in its natural state
The residues were mixed with 20 L of 20 mM HCl, 60 L of the sodium borate buffer provided with the AccQ Tag Ultra Derivatization Kit, and 20 L of 0.3% (w/v) AccQ acetonitrile solution and heated at 50 C for 10 min to obtain their AccQ derivatives
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