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Embryo sections or fixed cells were permeabilized with 0.2% Triton X-100 for 15 min at room temperature and blocked with 2.5% BSA for 1 h at room temperature
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Manfridi A, Forloni GL, Arrigoni-Martelli E, Mancia M
Time-Dependent Relationship Between Mucosal GSH Levels and Colitis Development To determine the role of GSH depletion induced by TNBS+ethanol on the appearance of injury, we determined the time dependent regulation of mucosal GSH levels and onset of injury in rats treated with TNBS+ethanol during the induction of colitis
It was a lot of trial and error for the young scientist
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