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Earlier studies largely focused on larger peptide-based GLP-1s, such as semaglutide, which were shown to suppress hunger by targeting appetite-regulating networks in the hypothalamus and hindbrain

The histopathologic study reported gastric and duodenal melanosis due to melanin deposits at the macrophage level of the lamina propria

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Moreover, biochemical analyses were performed on whole brain homogenates, assessing malondialdehyde, glutathione and catalase as oxidative stress markers using standard ELISA and spectrophotometric assays