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"A New Approach for Fast Metabolic Diagnostics in CMAMMA"
The PYR assay consists of three steps (Harrington et al., 2013): Biotinylated primers are used for PCR amplification and labeling. Streptavidin separates the PCR products and hybridizes to sequencing primers. Sequencing is performed, with nucleotides added one by one according to the template sequence
Mukherjee A, Malik H, Saha AP, Dubey A, Singhal DK, Boateng S, Saugandhika S, Kumar S, De S, Guha SK, Malakar D
Schiavoni, G., Lucente, M., Di Folca, A., Alessandri, N., Mongiardo, R., and Manzoli, U
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